Advancing STED super-resolution optical microscopy with differential depletion

Jong-Chan Lee^1,2*

¹New Biology, DGIST, Daegu, Korea
²New Biology Research Center, DGIST, Daegu, Korea

* Presenter:Jong-Chan Lee, email:jclee@dgist.ac.kr

In this presentation, we introduce two simple and easy-to-implement methods that can efficiently suppress the low spatial frequency background appearing in STED images. Polarization switching STED subtracts the background noise by obtaining background-only images by using switched polarization light beam. Differential stimulated emission depletion decomposes the background noise by altering the STED illumination intensities, and it achieves high signal-to-background ratio and spatial resolution at the same time.

Keywords: Super-resolution Optical Microscopy, Bioimaging, Biophysics