Multidisciplinary platforms to study biological questions

HSIUFANG Fan^1,2*

¹Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan
²Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan

* Presenter:HSIUFANG Fan, email:bendyfan@imst.nsysu.edu.tw

In our laboratory, we employ single-molecule techniques to study DNA metabolism, including site-specific recombination (Serine family vs. Serine family), RecA-mediated DNA repair (E. coli vs. D. Ficus), and nucleosome remodelling (Histone vs. Alba1). We also use fluorescence imaging to quantify RNA scaffolds in VLPs and explore protein-substrate binding. Recently, we utilized a single-cell imaging platform to investigate α-Synuclein diffusion in live cell lines and examined other amyloid proteins (Aβ and Human calcitonin) in vitro. These studies highlight the potential and significance of single-molecule/single-cell research in biology, shedding light on fundamental cellular behaviours and molecular mechanisms.

Keywords: single molecule assays, single cell assays, DNA metabolism, Amyloid protein