CRISPR Gene Editing: Transformative Applications in Biomedical Sensing

Ling-Shan Yu^1*

¹Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan

* Presenter:Ling-Shan Yu, email:lingshan0120@gmail.com

Gene editing technologies, the CRISPR/Cas systems, have revolutionized biomedical sensing by enabling rapid, sensitive, and specific detection of a wide range of pathogens and genetic markers. This talk explores the applications of CRISPR-based diagnostics in molecular sensing, highlighting recent advancements that leverage the versatility of these systems for point-of-care applications. This talk will introduce the fundamental design concepts of CRISPR technology and present both fluorescence-based and electrochemical platforms for sensitive and rapid diagnosis of infectious pathogens such as human hepatitis virus, human papillomavirus, and antimicrobial resistance markers. In addition, by implementing isothermal amplification approaches and utilizing Cas12a collateral cleavage to enhance detection signals, our platform significantly increases sensitivity and reduces diagnostic times. These enhancements offer potent tools to improve infection diagnostics. To facilitate practical applications, we have also developed a portable device that incorporates slip-chip technology and integrates artificial intelligence, enhancing the accessibility and usability of these diagnostic platforms in clinical settings. Overall, this talk will discuss the design, optimization, and potential clinical impact of these CRISPR-based diagnostic tools, emphasizing their role in advancing biomedical sensing through gene editing technology.

Keywords: CRISPR/Cas12, Isothermal amplification chemistry, biosensing, Infectious disease, Point-of-Care testing